mRNA Services

Residual double-stranded RNA (dsRNA) can form during the in vitro transcription process used to synthesize mRNA, potentially triggering unwanted immune responses in humans. To detect and quantify trace amounts of dsRNA contaminants, PackGene uses a double-antibody sandwich ELISA.

The process starts with coating the wells of an ELISA plate with a monoclonal or polyclonal antibody specific to dsRNA. When the mRNA sample is added, any dsRNA present binds to the capture antibody. After incubation, unbound material is washed away, and a second, labeled antibody—also specific to dsRNA—is introduced. This second antibody attaches to the bound dsRNA, forming a "sandwich" complex.

The detection antibody is typically linked to an enzyme, such as horseradish peroxidase (HRP), which reacts with a substrate to produce a measurable signal (colorimetric, fluorescent, or luminescent). The intensity of the signal is proportional to the dsRNA concentration and is quantified using a microplate reader, with values compared to a standard curve of known dsRNA concentrations. This highly specific and sensitive method ensures the safety and purity of mRNA therapeutics by detecting low levels of dsRNA contamination.

N1-methyl-pseudouridine (m1Ψ) is a modified nucleoside that plays several key roles when incorporated into mRNA:

• Enhanced Stability: mRNA with m1Ψ modifications demonstrates increased stability, prolonging the half-life and allowing for longer-lasting protein expression.
• Reduced Immunogenicity: m1Ψ reduces the immune response typically triggered by unmodified mRNA, preventing inflammation and degradation, making it more efficient for therapeutic delivery.
• Improved Translation Efficiency: m1Ψ enhances translation efficiency by promoting ribosome binding and the initiation of protein synthesis, resulting in higher protein output.
• Resistance to RNA Editing Enzymes: m1Ψ provides protection against RNA editing enzymes like ADAR, which can alter the mRNA sequence by editing adenosine residues. This ensures the integrity and fidelity of the mRNA sequence during protein synthesis.

Yes, we can subclone your ORF sequences into our proprietary vector, which includes the T7 promoter, UTR, and a 110A tail, for mRNA production.

Yes, we can supply cGMP mRNA through our partnership with Kudo Biotechnology. For more information, please visit our GMP mRNA service webpage. Please check our GMP mRNA webpage.